Diagnostic Services Rate Schedule (updated 3-4-2015)
The Florida Extension Plant Diagnostic Center (FEPDC) is a fee-based facility provided by the University of Florida-IFAS, Plant Pathology Department. Fees collected by FEPDC are used to pay for diagnostic supplies and equipment as well as funding the lab assistants who process the samples.
Standard base charge, in-state: $40.00 (out-of-state: $45.00, international*: $125.00) – includes sample dissection and observation, basic dissecting and light microscopy, culturing on semi-selective media, 1 immunostrip if needed, diagnostic report, and management recommendations.
Standard base charge, Rapid Turfgrass Diagnostic Service: $75.00 for US (international*: $300.00) – includes sample dissection and observation, basic dissecting and light microscopy, culturing on semi-selective media, preliminary report, final diagnostic report, and management recommendations.
*International services – International diagnostic service requires prenotification. Per our permits, samples shipped directly to the lab without prenotification and proper shipping labels must be destroyed without opening the package. Call/email for availability and permits/forms: (352) 392-1795 or firstname.lastname@example.org. International diagnostic service includes sample dissection and observation, basic dissecting and light microscopy, culturing on semi-selective media, 1 immunostrip or conventional PCR assay if needed, and diagnostic report. This service is offered via prenotification only. Additional ELISA, PCR, Biolog, sequencing, or other tests will be charged on top of the base charge after consultation with the client.
Specific tests we offer in-house as needed and upon request:
- Serology/ELISA: Charged at market rate (on top of the base charge if a full diagnostic workup is requested); often the cost is around $20 per sample. The total charge may change depending on the number of samples and the cost of the ELISA kit. Current tests include Sugarcane mosaic virus (SCMV), Blueberry shock virus, and Potyvirus group. When ELISA-based immunostrips are available, these may be used for a battery of tests as needed (can include Cymbidium mosaic virus and Odontoglossum ringspot virus, Potato virus Y (PVY), Impatiens necrotic spot virus (INSV), Squash mosaic virus (SqMV), Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV), Acidovorax avenae (bacterial fruit blotch), Phytophthora spp., Ralstonia spp., Xanthomonas axonopodis (citrus canker), etc.)
- Biolog: Charged at $16 per isolate if pure bacterial cultures are provided by the client. Or charged on top of base charge, after consultation and approval by client, if needed during full diagnostic workup.
- PCR: Charged at the molecular rate of $35 per sample for conventional PCR and $65 for real-time PCR (qPCR) and reverse-transcriptase PCR (RT-PCR). Add $5 for each out-of state sample. These tests are conducted at additional cost after consultation with the client following full diagnostic workup, or are stand-alone at the request of the client. These tests are offered with the result of detected/not detected. Please call for a list of protocols; this list is growing rapidly and currently includes general fungal ITS and general bacterial 16S (conventional PCR amplification for sequencing), Xylella fastidiosa (bacterial leaf scorch), Blueberry Red Ringspot Virus, Clavibacter michigenensis subsp. michigenensis (tomato bacterial canker), phytoplasmas and Fusarium wilt of palms, Ca. Liberibacter asiaticus (citrus greening), Phytophthora ramorum (sudden oak death), Ralstonia solanacearum (southern wilt), Rafaella lauricola (laurel wilt), and others.
- Sequencing: We can amplify and clean DNA, sequence it, and analyze the resulting data using NCBI BLAST. PCR is charged at the above conventional PCR rates. Cleanup and sequencing (one run, two directions) is $25 per sample. BLAST analysis is $10 per sample. Client is responsible for indicating all services to be performed and for selecting the primers to be used. If no specific primers are indicated, the lab will perform the PCR and sequencing using species-specific (if we have them) or general ITS or 16S primers, as appropriate, and will conduct the BLAST analysis.
UF Plant Diagnostic Center
Building 1291, 2570 Hull Road
Gainesville, FL 32611-0830