| Research program |
Year 1 |
Year 2 |
Year 3 |
| |
| Develop rapid, robust, and reliable diagnostic assays |
|
|
|
| Make and screen monoclonal antibodies |
X |
|
|
| Purify and identify cell-surface proteins |
X |
|
|
| Bioinformatics to identify R3b2-specific primer sets |
X |
|
|
| Exhaustive final screening of candidate primers and antibodies |
|
X |
|
| Develop nanodetection conjugation and bioassays |
X |
X |
|
| |
|
|
|
| |
|
|
|
| Identify R3b2 genes involved in cold adaptation and growth in plant hosts |
|
|
|
| Extract pathogen mRNA & probe microarrays |
X |
|
|
| Identify, mutate, and analyze candidate cold/potato genes |
X |
X |
X |
| |
|
|
|
| Educational & outreach program |
Year 1 |
Year 2 |
Year 3 |
| |
|
|
|
| Develop a package of optimized education and management training modules |
|
|
|
| Initial workshop & baseline surveys, management plan |
X |
|
|
| Develop curriculum content and design modules |
X |
X |
|
| Workshops and training of target audiences |
|
X |
X |
| Validate diagnostic/monitoring protocols offshore |
|
X |
|
| Ring-testing and adaptation of diagnostic protocols |
|
X |
X |
| Final assessments; write reports and research publications |
|
|
X |
| |
|
|
|
