About
My primary research interests include characterization of host-pathogen interactions and understanding molecular physiology of biotic (and abiotic) stress tolerance in crops. As a member of Profs. Jeff Jones’ and Frank White’s research programs, we seek to functionally characterize Ca. Liberibacter asiaticus and Ca. L. solanacearum effectors using transgenic approaches. I am also interested in developing marker-free gene and base editing protocols for Clavibacter nebraskensis.
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Education
Ph.D. (Plant Biochemistry), 1997, Jawaharlal Nehru University, India
M.Sc. (Life Sciences), 1991, Jawaharlal Nehru University, India
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Research
Base editing in Clavibacter nebraskensis
Characterization of Ca. Liberibacter effectors
Liberibacter crescens as a surrogate host for functional genomics of uncultured Ca. Liberibacters.
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Publications
Cai, L., Jain, M., Munoz-Bodnar, A., Huguet-Tapia, J. C., Gabriel, D. W. A synthetic ‘essentialome’ for axenic culturing of Candidatus Liberibacter asiaticus. BMC Res. Notes (accepted).
Jain, M., Cai, L., Black, I., Azadi, P., . Carlson, R. W., Jones, K. M., and Gabriel, D. W. ‘Candidatus Liberibacter asiaticus’-encoded BCP peroxiredoxin suppresses lipopolysaccharide-mediated defense signaling and nitrosative stress in planta. Plant-Microbe Interact. (2021) https://doi.org/10.1094/MPMI-09-21-0230-R.
Black, I., Heiss, C., Jain, M., Muszynski, , Carlson, R. W., Gabriel, D. W., and Azadi, P. Structure of from Liberibacter crescens lipopolysaccharide is low molecular weight and offers insight into Candidatus Liberibacter biology. Int. J. Mol. Sci. (2021) 22:11240.
Cai, L., Jain, M., Sena-Velez, M., Jones, K. M., Fleites, L. A., M. Heck, and Gabriel, D. W. Tad pilus-mediated twitching motility is essential for DNA uptake and survival in all Liberibacters. PLoS One (2021) 16:e0258583.
Sena-Velez, M., Holland, S., Aggarwal, M., Cogan, N., Jain, M., Gabriel, D. W., and Jones, K. Growth dynamics and survival of Liberibacter crescens BT-1, an important model organism for the citrus Huanglongbing pathogen 'Candidatus Liberibacter asiaticus'. Environ. Microbiol. (2019) 85:e01656-19.
Jain, M., Cai, L., Munoz-Bodnar, A., Fleites, L. A., Davis, M. J., and Gabriel, D. W. Liberibacter crescens is a cultured surrogate for functional genomics of uncultured pathogenic ‘Ca. Liberibacter’ spp. and is naturally competent for transformation. (2019) 109:1811-1819.
Merfa, M. V., Pérez-López, E., Naranjo, E., Jain, M., Gabriel, D. W., and De La Fuente, L. Progress and obstacles in culturing ‘Candidatus Liberibacter asiaticus’, the bacterium associated with Huanglongbing (HLB). (2019) 109:1092-1101.
Naranjo, E., Merfa, M. V., Ferreira, V., Jain, M., Davis, M. J., Bahar, O., Gabriel, D. W., and De La Fuente, L. Liberibacter crescens biofilm formation in vitro: establishment of a model system for pathogenic ‘Candidatus Liberibacter spp.’ Rep. (2019) 9:5150.
Jain, M., Munoz-Bodnar, A., and Gabriel, D.W. ‘Candidatus Liberibacter asiaticus’ peroxiredoxin (LasBCP) suppresses oxylipin-mediated defense signaling in citrus. Plant Physiol. (2019) 236:61-65
Zhang, S., Jain, M., Fleites, L. A., Rayside, P. A. and Gabriel, D. W. Identification and characterization of menadione and benzethonium chloride as potential treatments of Pierce’s Disease of grapevines. Phytopathol. (2019) 109:233-239.
Jain, M., Munoz-Bodnar, A., Zhang, S. and Gabriel, D. W. A secreted ‘Candidatus Liberibacter asiaticus’ peroxiredoxin simultaneously suppresses both localized and systemic innate immune responses in planta. Plant-Microbe Interact. (2018) 31:1312-1322.
Hsieh, Y. F., Jain, M., Wang, J. and Gallo, M. Direct organogenesis from cotyledonary node explants suitable for Agrobacterium-mediated transformation in peanut (Arachis hypogaea). Plant Cell Tissue Organ Cult. (2017) 128:161-175.
Jain, M., Munoz-Bodnar, A. and Gabriel, D. W. Concomitant loss of the glyoxalase system and glycolysis makes the uncultured pathogen “Candidatus Liberibacter asiaticus” an energy scavenger. Environ. Microbiol. (2017) 83:e01670-17.
Jain, M., Fleites, L. A. and Gabriel, D. W. A small Wolbachia protein directly represses phage lytic cycle genes in “Candidatus Liberibacter asiaticus” within psyllids. mSphere (2017) 2:e00171-17.
Devi, M. J., Sinclair, T. R., Jain, M. and Gallo, M. Leaf aquaporin transcript abundance in peanut genotypes diverging in expression of the limited‐transpiration trait when subjected to differing vapor pressure deficits and aquaporin inhibitors. Planta. (2016) 156:387-396.
Jain, M., Fleites, L. A. and Gabriel, D. W. Prophage-encoded peroxidase in ‘Candidatus Liberibacter asiaticus’ is a secreted effector that suppresses plant defenses. Plant-Microbe Interact. (2015) 28:1330-1337.
Jain M., Gallo M., Chengalrayan K., Shaikh N. P., MacDonald G. E., and Davis J. M. The insecticide phorate-induced host defense responses condition acquired resistance to tomato spotted wilt in peanut (Arachis hypogaea). J. Phytopathol. (2015) 163:853-866.
Rustogi, A., Jain, S., Kumar, D., Shekhar, S., Jain, M., Bhat, V., and Sarin, N. B. High efficiency transformation of banana [Musa acuminata cv. Matti (AA)] for enhanced tolerance to salt and drought stress through overexpression of a peanut Salinity-Induced Pathogenesis-Related class 10 protein. Mol. Biotechnol. (2015) 57:27-35.
Fleites, L. A., Jain, M., Zhang, S., and Gabriel, D. W. “ Liberibacter asiaticus” prophage late genes may limit host range and culturability. Appl. Environ. Microbiol. (2014) 80:6023-6030.
Joshi, S., Jain, M., Tillman, B. L., Altpeter, F., and Gallo, M. Comparative analysis of a direct plant regeneration protocol from immature leaf whorl and floral explants for three elite US sugarcane (Saccharum hybrid) cultivars. In Vitro Cell Dev. Biol. -Plant (2013) 49:674-681.
Pathak, B. P., Jain, M., Tillman, B. L., Grusak, M. A., and Gallo, M. Effect of gypsum application on mineral composition in peanut pod walls and seeds. Crop Sci. (2013) 53:1658-1667.
Jain, S., Kumar, D., Jain, M., Chaudhary, P., Deswal, R., and Sarin, N. B. 2012. Ectopic overexpression of a novel salt stress-induced pathogenesis-related class 10 protein (PR10) gene from peanut (Arachis hypogaea) affords broad spectrum abiotic stress tolerance in transgenic tobacco. Plant Cell Tissue Organ Cul. (2012) 109:19-31.
Jain, M., Pathak, B. P., Harmon, A. C., Tillman, B. L., and Gallo, M. Calcium dependent protein kinase (CDPK) expression during fruit development in cultivated peanut (Arachis hypogaea) under Ca2+-sufficient and -deficient growth regimens. Plant Physiol. (2011) 168:2272-2277.
Rijavec, T., Jain, M., Dermastia, M., and Chourey, P.S. Spatial and temporal profiles of cytokinin biosynthesis and accumulation in developing caryopses of maize. Bot. (2010) 107:1235-1245.
Tirumalaraju, S. V., Jain M., and Gallo, M. Differential gene expression in roots of nematode-resistant and -susceptible peanut (Arachis hypogaea) cultivars in response to early stages of root-knot nematode (Meloidogyne arenaria) parasitization. Plant Physiol. (2010) 168:481-492.
Jain, M., Chourey, P. S., Boote, K. J., and Allen, L. H. Jr. Short-term high temperature growth conditions during vegetative-to-reproductive phase transition irreversibly compromise cell wall invertase-mediated sucrose catalysis and microspore meiosis in grain sorghum (Sorghum bicolor). Plant Physiol. (2010) 167:578-582.
Jain, M., Li, Q.-B., and Chourey, P. S. Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition. Planta. (2008) 134:161-173.
Jain, M., Chourey, P. S., Li, Q.-B., and Pring, D. R. Expression of cell wall invertase and several other genes of sugar metabolism in relation to seed development in sorghum (Sorghum bicolor). Plant Physiol. (2008) 165:331-344.
Jain, M., Prasad, P. V. V., Boote, K.J., Allen, L.H. Jr., and Chourey, P.S. Effects of season-long high temperature growth conditions on sugar-to-starch metabolism in developing microspores of grain sorghum (Sorghum bicolor Moench). Planta (2007) 227:67-79.
Jain, M., Chengalrayan, K., Abouzid, A., and Gallo, M. Prospecting PMI/mannose selection system for the recovery of transgenic sugarcane (Saccharum hybrid) plants. Plant Cell Rep. (2007) 26:581-590.
Chourey, P. S., Jain, M., Li, Q.-B., and Carlson, S. J. Genetic control of cell wall invertases in developing endosperm of maize. Planta (2006) 223:159-167.